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Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 314-320 doi: 10.15302/J-FASE-2014038

摘要： This study was conducted to systematically assess the reproductive performance of transgenic ewes. In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the transgene did not affect the reproductive performance in sheep.

关键词： TLR4 transgenic ewe     safety assessment     reproductive trait     oocyte     DNA methylation    

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Meta-analysis of the risk factors of breast cancer concerning reproductive factors and oral contraceptive

Qiong DAI MD, Bei LIU MD, Yukai DU MM,

《医学前沿（英文）》 2009年 第3卷 第4期   页码 452-458 doi: 10.1007/s11684-009-0080-z

摘要： The authors performed a meta-analysis of case-control studies that addressed whether reproductive factors and oral contraceptive use were associated with breast cancer by searching the MEDLINE, PubMed, Proquest, Embase, ScienceDirect, African Healthline, BMJ Health Intelligence and Chinese Periodical net databases for all English-language and Chinese-language papers published from January 1, 1997 to December 31, 2007. A total of 15 studies calculating pool ORs indicated that menopausal age >50yr [odds ratio (OR), 1.39; 95% confidence interval (CI), 1.22―1.57] and oral contraceptive use (OR, 2.12*, “*”: summary OR was adjusted; 95% CI, 1.24―3.62) were correlated with the increase in breast cancer risk while the summary OR based on number of full-term pregnancies ≥1 (OR, 0.63*; 95% CI, 0.60―0.68) and breast-feeding (OR, 0.76; 95% CI, 0.64―0.90) indicated no association with breast cancer risk. The correlation was statistically significant. Menopausal age >50yr and oral contraceptive use are positively correlated with an increase in breast cancer risk while breast-feeding and number of full-term pregnancies ≥1 are protective factors.

关键词： meta-analysis     breast cancer     risk factors     reproductive factors     oral contraceptive use    

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Top-geoherbs of traditional Chinese medicine: common trait, quality characteristics and formation

Luqi Huang, Lanping Guo, Chaoyi Ma, Wei Gao, Qingjun Yuan

《医学前沿（英文）》 2011年 第5卷 第2期   页码 185-194 doi: 10.1007/s11684-011-0141-y

摘要： Top-geoherbs used in China are always featured with high qualities, and they grow in specified areas with specific environment. Recently, researches on top-geoherbs have attracted increasing attention in China and other countries. In order to have a thorough knowledge of top-geoherbs, this article reviews the concept, historical evolution, common trait and quality characteristics of top-geoherbs, and explains the forming mechanism including genetic mechanism and environmental mechanism. In addition, it introduces the influence of human factors on the quality of top-geoherbs. Finally, it proposes some problems that should be paid attention to in the researches on top-geoherbs.

关键词： top-geoherbs     common trait     quality characteristics     formation     biological mechanism    

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THE ROLE OF LONG-TERM EXPERIMENTS IN VALIDATING TRAIT-BASED APPROACHES TO ACHIEVING MULTIFUNCTIONALITY

《农业科学与工程前沿（英文）》 2022年 第9卷 第2期   页码 187-196 doi: 10.15302/J-FASE-2021438

摘要：

Quantifying the relationships between plant functional traits and ecosystem services has been promoted as an approach to achieving multifunctional grassland systems that balance productivity with other regulating, supporting and cultural services. Establishing trade-offs and synergies between traits and services has largely relied on meta-analyses of studies from different systems and environments. This study demonstrated the value of focused studies of long-term experiments in grassland systems that measure traits and services in the same space and time to better understand the ecological constraints underlying these trade-offs and synergies. An analysis is presented that uses data from the Park Grass Experiment at Rothamsted Research on above-ground productivity, species richness and soil organic carbon stocks to quantify the relationships between these three outcomes and the power of variance in plant functional traits in explaining them. There was a trade-off between plots with high productivity, nitrogen inputs and soil organic carbon and plots with high species richness that was explained by a functional gradient of traits that are indicative of contrasting strategies of resource acquisition of resource conservation. Examples were identified of using functional traits to identify opportunities for mitigating these trade-offs and moving toward more multifunctional systems.

关键词： multifunctional grassland systems / Park Grass Experiment / soil organic carbon / ecosystem service    

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Cell therapy for the treatment of reproductive diseases and infertility: an overview from the mechanism

《医学前沿（英文）》 2022年 第16卷 第6期   页码 827-858 doi: 10.1007/s11684-022-0948-8

摘要： Infertility is experienced by 8%–12% of adults in their reproductive period globally and has become a prevalent concern. Besides routine therapeutic methods, stem cells are rapidly being examined as viable alternative therapies in regenerative medicine and translational investigation. Remarkable progress has been made in understanding the biology and purpose of stem cells. The affected pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) are further studied for their possible use in reproductive medicine, particularly for infertility induced by premature ovarian insufficiency and azoospermia. Accordingly, this study discusses current developments in the use of some kinds of MSCs such as adipose-derived stem cells, bone marrow stromal cells, umbilical cord MSCs, and menstrual blood MSCs. These methods have been used to manage ovarian and uterine disorders, and each technique presents a novel method for the therapy of infertility.

关键词： infertility     stem cell therapy     mesenchymal stem cells     pluripotent stem cells    

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Functional trait differences between native bunchgrasses and the invasive grass

Huiqin HE, Thomas A. MONACO, Thomas A. JONES

《农业科学与工程前沿（英文）》 2018年 第5卷 第1期   页码 139-147 doi: 10.15302/J-FASE-2017175

摘要： We conducted 30- and 60-d greenhouse experiments to compare functional traits of (invasive annual grass) and four perennial bunchgrasses under well-watered or drought conditions. Even under drought, experienced significantly less stress (i.e., higher xylem pressure potential and greater shoot water content, water use per day and water-use efficiency) and biomass production than the perennial grasses after 30 d. However, after 60 d, its superiority was reduced under infrequent watering. Differences among perennial grasses were more pronounced for physiological traits under infrequent watering and for morphological traits under frequent watering. (fast-growing species) had a higher transpiration rate, lower leaf temperature, and lower water-use efficiency than the other grasses after 30 d. In contrast, (slow-growing) had lower xylem pressure potential and higher leaf temperature than all other grasses under infrequent watering. Under frequent watering, shoot dry mass and specific leaf area of was matched by (moderate-growing species). Our results indicate that multiple-species plantings or seedings are necessary to foster greater weed resistance against . We also emphasize that when choosing plant material for restoration, performance during both pulse (resource-rich) and inter-pulse (resource-poor) periods should be considered.

关键词： annual grass     comparative growth     drought response     invasive plant     native grass     specific leaf area     soil-water use    

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Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive

Mengmeng ZHAO,Juanjuan QIAN,Jiexiong XIE,Tiantian CUI,Songling FENG,Guoqiang WANG,Ruining WANG,Guihong ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 153-160 doi: 10.15302/J-FASE-2016097

摘要： Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases impacting the swine industry and is characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages. The nonstructural protein 9 gene, , encoding the RNA-dependent RNA polymerase, is generally regarded as fairly conserved when compared to other viral proteins. Antibodies against 9 will be of great importance for the diagnosis and treatment of the causal agent, PRRS virus. A study was undertaken to generate polyclonal antibodies against the immunodominant 9. For this purpose, the 9 was expressed in and subsequently used as an antigen to immunize New Zealand rabbits. Antiserum was identified via an indirect ELISA, and then verified based on the ability to react with both naturally and artificially expressed 9. Results of virus neutralization test showed that this antiserum could not neutralize the PRRSV. Nevertheless, this antiserum as a diagnostic core reagent should prove invaluable for further investigations into the mechanism of PRRS pathogenesis.

关键词： PRRSV     Nsp9     expression     antibody     neutralize    

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Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

《农业科学与工程前沿（英文）》 2014年 第1卷 第2期   页码 91-95 doi: 10.15302/J-FASE-2014005

摘要： Backfat thickness is a good predictor of carcass lean content, an economically important trait, and a main breeding target in pig improvement. In this study, the candidate genes and genomic regions associated with the tenth rib backfat thickness trait were identified in two independent pig populations, using a genome-wide association study of porcine 60K SNP genotype data applying the compressed mixed linear model (CMLM) statistical method. For each population, 30 most significant single-nucleotide polymorphisms (SNPs) were selected and SNP annotation implemented using Build 10.2. In the first population, 25 significant SNPs were distributed on seven chromosomes, and SNPs on SSC1 and SSC7 showed great significance for fat deposition. The most significant SNP (ALGA0006623) was located on SSC1, upstream of the gene. In the second population, 27 significant SNPs were recognized by annotation, and 12 SNPs on SSC12 were related to fat deposition. Two haplotype blocks, M1GA0016251-MARC0075799 and ALGA0065251-MARC0014203-M1GA0016298-ALGA0065308, were detected in significant regions where the and genes were identified as contributing to fat metabolism. The results indicated that genetic mechanism regulating backfat thickness is complex, and that genome-wide associations can be affected by populations with different genetic backgrounds.

关键词： backfat thickness     SNP chip     genome-wide association study     compressed mixed linear model     pig    

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不同民族流动育龄妇女的避孕状况分析

张君娴,杨婷,徐晓帆,龙春燕,刘建苏,李莉

《中国工程科学》 2015年 第17卷 第6期   页码 57-60

摘要：

了解乌鲁木齐市流动育龄妇女的避孕状况，为今后开展避孕方法知情选择优质服务提供参考。采用分层-整群-随机抽样的方法，对18~50岁符合条件的对象进行问卷调查，主要包括人口学特征、避孕措施的使用情况等。采用卡方检验和多因素Logistic回归方法进行统计分析。本次调查的106 568例流动育龄妇女中，避孕方法使用比例为92.78 %，避孕方法的选择依次为宫内节育器（65.74 %）、药物（23.17 %，包括口服避孕药、皮下埋植剂、避孕针等含药避孕方法）、避孕套（9.06 %）、绝育术（2.03 %）。多因素Logistic回归分析显示民族、学历、职业、婚姻状况和性生活频率对避孕方法的使用有影响。应根据不同民族的情况和流动育龄妇女的人口学特征，提供有针对性和个性化的避孕方法知情选择指导和服务。

关键词： 流动育龄妇女     避孕状况     分析    

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A brief review of microRNA and its role in PRRSV infection and replication

Xuekun GUO,Wenhai FENG

《农业科学与工程前沿（英文）》 2014年 第1卷 第2期   页码 114-120 doi: 10.15302/J-FASE-2014022

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV), a single-stranded RNA virus, mainly infects cells of monocyte/macrophage lineage. Recently, host microRNAs were shown to be capable of modulating PRRSV infection and replication by multiple ways such as targeting viral genomic RNA, targeting viral receptor and inducing antiviral response. MicroRNAs are small RNAs and have emerged as important regulators of virus-host cell interactions. In this review, we discuss the identified functions of host microRNAs in relation to PRRSV infection and propose that cellular microRNAs may have a substantial effect on cell or tissue tropism of PRRSV.

关键词： porcine reproductive and respiratory syndrome virus (PRRSV)     microRNA     antiviral     viral tropism    

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proxies for yield potential and heat stress tolerance in spring wheat: heritability estimates and marker-trait

Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS

《农业科学与工程前沿（英文）》 2019年 第6卷 第3期   页码 296-308 doi: 10.15302/J-FASE-2019269

摘要：

The application of spectral reflectance indices (SRIs) as proxies to screen for yield potential (YP) and heat stress (HS) is emerging in crop breeding programs. Thus, a comparison of SRIs and their associations with grain yield (GY) under YP and HS conditions is important. In this study, we assessed the usefulness of 27 SRIs for indirect selection for agronomic traits by evaluating an elite spring wheat association mapping initiative (WAMI) population comprising 287 elite lines under YP and HS conditions. Genetic and phenotypic analysis identified 11 and 9 SRIs in different developmental stages as efficient indirect selection indices for yield in YP and HS conditions, respectively. We identified enhanced vegetation index (EVI) as the common SRI associated with GY under YP at booting, heading and late heading stages, whereas photochemical reflectance index (PRI) and normalized difference vegetation index (NDVI) were the common SRIs under booting and heading stages in HS. Genome-wide association study (GWAS) using 18704 single nucleotide polymorphisms (SNPs) from Illumina iSelect 90K identified 280 and 43 marker-trait associations for efficient SRIs at different developmental stages under YP and HS, respectively. Common genomic regions for multiple SRIs were identified in 14 regions in 9 chromosomes: 1B (60–62 cM), 3A (15, 85–90, 101–105 cM), 3B (132–134 cM), 4A (47–51 cM), 4B (71–75 cM), 5A (43–49, 56–60, 89–93 cM), 5B (124–125 cM), 6A (80–85 cM), and 6B (57–59, 71 cM). Among them, SNPs in chromosome 5A (89–93 cM) and 6A (80–85 cM) were co-located for yield and yield related traits. Overall, this study highlights the utility of SRIs as proxies for GY under YP and HS. High heritability estimates and identification of marker-trait associations indicate that SRIs are useful tools for understanding the genetic basis of agronomic and physiological traits.

关键词： genome-wide association study (GWAS)     heat tolerance     spectral reflectance     spring wheat    

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乌鲁木齐市流动育龄妇女阴道炎患病现况调查

张君娴,周彦岭,刘建苏,王珊,李莉,梅桂,李琳,巴哈古丽·塔吉曼提

《中国工程科学》 2014年 第16卷 第5期   页码 85-89

摘要：

了解乌鲁木齐市流动人口育龄妇女阴道炎感染患病现况，为今后的生殖道感染防治提供参考依据。本文采取分层-整群-抽样的方法，在乌鲁木齐市六区一县流动人口较集中的社区，由经过培训的医生对18～50 岁的流动育龄妇女进行生殖道感染问卷调查、妇科检查与实验室检查。本研究共检查106 568 名符合条件的流动妇女，其中细菌性阴道炎的患病率为16.15 %；滴虫性阴道炎的患病率为12.66 %；霉菌性阴道炎的患病率为4.52 %。3 种阴道炎的患病率为19.84 %。应根据不同民族的特点，采取一定的防治措施，降低流动育龄妇女的阴道炎患病率，特别是对文化程度为文盲或小学、职业为农民、月收入在1 500～1 599 元的对象，应加大干预的力度。

关键词： 流动育龄妇女     阴道炎     患病现况    

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Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine reproductive

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 65-71 doi: 10.15302/J-FASE-2016086

摘要： Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failures in sows and respiratory diseases in pigs of all ages. PRRS virus (PRRSV) is its causative agent and has caused huge economic losses in the swine industry. Porcine sialoadhesin (pSn) is a putative receptor of PRRSV. Previous studies have shown that a pSn V-set Ig-like domain is significant in PRRSV infection. However, its structural details are not fully known, hindering our deep understanding of PRRSV infection. In this study, we successfully cloned, expressed and purified the pSn V-set Ig-like domain in S2 cells. Then we tried to crystallize the target protein and predicted its structure. This will establish the foundation for the further structural study of pSn, deepen our understanding of the invasion mechanism of PRRSV, and support the structural information for the development of clinical drugs and vaccines against PRRSV.

关键词： PRRSV     porcine sialoadhesin     V-set Ig-like domain     Drosophila S2 cell     crystallization    

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Reproductive toxicity study with a novel deoxyguanosine analogue (Metacavir) in pregnant SD rats

null

《医学前沿（英文）》 2015年 第9卷 第1期   页码 82-89 doi: 10.1007/s11684-015-0376-0

摘要：

Our preliminary studies demonstrated that Metacavir has potential to become a new anti-HBV agent. The main targets of the toxic effects of Metacavir, in rhesus monkeys, were gastrointestinal tracts, liver, blood, and kidneys, which were not related to mitochondrial effects. In this study, the maternal toxicity, embryo-fetal developmental toxicity and teratogenicity were studied in pregnant Sprague-Dawley rats after intragastric administration of Metacavir (200, 100, 50, 0 mg/kg body weight) during the first 6–15 days of pregnancy. Slower weight gain was observed in 5 out of 21 rats subjected to a 200 mg/kg dose, as well as 2 out of 20 subjected to a 100 mg/kg dose. Compared with the solvent control group, the calibration weight gain in the 200 mg/kg and 100 mg/kg dosage groups respectively, during first 6–20 pregnant days were significantly different (P<0.01, P<0.05). Significant dose related adverse effects to other reproductive parameters were not seen in F0 and F1, but the number of stillbirths in high dose group showed notably difference compared with the control group (P<0.05), while the litter incidence showed no difference. No Metacavir-associated pathological changes were observed. The present research indicated that at a dose of 200 mg/(kg·d) (i.e., 40 times the effective dose in rats), Metacavir shows some maternal toxicity to SD rats. The embryotoxicity in the 200 mg/kg group encompass decreased fetal body weight, and higher fetal mortality rates, compared with the control group. However, the litter incidence showed no statistical difference. All the treated rats displayed normal bone development, no teratogenicity and without adverse effects on fetal development, thus indicating that below a dose of 200 mg/(kg·d) there is no teratogenic side effects.

关键词： deoxyguanosine analogue     Metacavir     pregnancy     maternal toxicity     embryo toxicity     teratogenicity    

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Genetic study and molecular breeding for high phosphorus use efficiency in maize

null

《农业科学与工程前沿（英文）》 2019年 第6卷 第4期   页码 366-379 doi: 10.15302/J-FASE-2019278

摘要：

Phosphorus is the second most important macronutrient after nitrogen and it has many vital functions in the life of plants. Most soils have a low available P content, which has become a key limiting factor for increasing crop production. Also, low P use efficiency (PUE) of crops in conjunction with excessive application of P fertilizers has resulted in serious environmental problems. Thus, dissecting the genetic architecture of crop PUE, mining related quantitative trait loci (QTL) and using molecular breeding methods to improve high PUE germplasm are of great significance and serve as an efficient approach for the development of sustainable agriculture. In this review, molecular and phenotypic characteristics of maize inbred lines with high PUE, related QTL and genes as well as low-P responses are summarized. Based on this, a breeding strategy applying genomic selection as the core, and integrating the existing genetic information and molecular breeding techniques is proposed for breeding high PUE maize inbred lines and hybrids.

关键词： maize     phosphorus use efficiency     quantitative trait loci     genetic study     molecular breeding     genomic selection    

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标题 作者 时间 类型 操作

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

期刊论文

Meta-analysis of the risk factors of breast cancer concerning reproductive factors and oral contraceptive

Qiong DAI MD, Bei LIU MD, Yukai DU MM,

期刊论文

Top-geoherbs of traditional Chinese medicine: common trait, quality characteristics and formation

Luqi Huang, Lanping Guo, Chaoyi Ma, Wei Gao, Qingjun Yuan

期刊论文

THE ROLE OF LONG-TERM EXPERIMENTS IN VALIDATING TRAIT-BASED APPROACHES TO ACHIEVING MULTIFUNCTIONALITY

期刊论文

Cell therapy for the treatment of reproductive diseases and infertility: an overview from the mechanism

期刊论文

Functional trait differences between native bunchgrasses and the invasive grass

Huiqin HE, Thomas A. MONACO, Thomas A. JONES

期刊论文

Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive

Mengmeng ZHAO,Juanjuan QIAN,Jiexiong XIE,Tiantian CUI,Songling FENG,Guoqiang WANG,Ruining WANG,Guihong ZHANG

期刊论文

Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

期刊论文

不同民族流动育龄妇女的避孕状况分析

张君娴,杨婷,徐晓帆,龙春燕,刘建苏,李莉

期刊论文

A brief review of microRNA and its role in PRRSV infection and replication

Xuekun GUO,Wenhai FENG

期刊论文

proxies for yield potential and heat stress tolerance in spring wheat: heritability estimates and marker-trait

Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS

期刊论文

乌鲁木齐市流动育龄妇女阴道炎患病现况调查

张君娴,周彦岭,刘建苏,王珊,李莉,梅桂,李琳,巴哈古丽·塔吉曼提

期刊论文

Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine reproductive

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

期刊论文

Reproductive toxicity study with a novel deoxyguanosine analogue (Metacavir) in pregnant SD rats

null

期刊论文

Genetic study and molecular breeding for high phosphorus use efficiency in maize

null

期刊论文